Targeted Transcriptome and KRAS Mutation Analysis Improve the Diagnostic Performance of EUS-FNA Biopsies in Pancreatic Cancer.

Joanne LundyHugh GaoWilliam BerrySamar Masoumi-MoghoddamBrendan J JenkinsDaniel G Croagh

Published in: Clinical cancer research : an official journal of the American Association for Cancer Research (2021)

Our findings support snap-frozen EUS-FNA biopsies as a feasible tissue source for the integrated genomic and transcriptomic analysis of patients presenting with PDAC from all tumor stages, including cases with nondiagnostic cytology. Our transcriptome-derived genetic signature in combination with tissue KRAS mutation analysis significantly improves upon the diagnostic accuracy of current standard procedures, and has potential clinical utility in improving the speed and accuracy of diagnosis for patients presenting with PDAC.

Keyphrases

fine needle aspiration
ultrasound guided
single cell
rna seq
genome wide
gene expression
copy number
high grade
cancer therapy
climate change