Facile Design of Phase Separation for Microfluidic Droplet-Based Liquid Phase Microextraction as a Front End to Electrothermal Vaporization-ICPMS for the Analysis of Trace Metals in Cells.
Xiaoxiao YuBeibei ChenMan HeHan WangSongbai TianBin HuPublished in: Analytical chemistry (2018)
The issue of quantifying trace metals in cells has drawn widespread attention but is threatened with insufficient sensitivity of the instruments, complex cellular matrix and limited cell consumption. In this study, microfluidic droplet-based liquid phase microextraction (LPME), as a miniaturized platform, was developed and combined with electrothermal vaporization (ETV)-inductively coupled plasma mass spectrometry (ICPMS) for the analysis of trace Cd, Hg, Pb, and Bi in cells. A novel and facile design of phase separation region was proposed, which made the phase separation very easily for subsequent ETV-ICPMS detection. Mechanism of the phase separation was carefully discussed using the incompressible formulation of the Navier-Stokes equations. The developed microfluidic droplet-based LPME system exhibited much higher extraction efficiency to target metals than microfluidic stratified flow-based LPME. Under the optimized conditions, the limits of detection of the proposed microfluidic droplet-based LPME-ETV-ICPMS system were 2.5, 3.9, 5.5, and 3.4 ng L-1 for Cd, Hg, Pb, and Bi, respectively. The accuracy of the developed method was well validated by analyzing the target metals in Certified Reference Materials of GBW07601a human hair. Finally, the proposed method was successfully applied to the analysis of target metals in HeLa and HepG2 cells with the recoveries for the spiked samples ranging from 83.5 to 112.3%. Overall, the proposed design is a simple and reliable solution for the phase separation on droplet-chip and the microfluidic droplet-based LPME-ETV-ICPMS combination strategy shows great promise for trace elements analysis in cells.
Keyphrases
- high throughput
- single cell
- induced apoptosis
- circulating tumor cells
- cell cycle arrest
- acute lymphoblastic leukemia
- heavy metals
- label free
- health risk assessment
- health risk
- ionic liquid
- endoplasmic reticulum stress
- signaling pathway
- endothelial cells
- cell death
- fluorescent probe
- machine learning
- simultaneous determination
- gas chromatography
- artificial intelligence
- liquid chromatography tandem mass spectrometry
- single molecule
- tandem mass spectrometry
- nk cells
- data analysis