Rational protein design of Bacillus sp. MN chitosanase for altered substrate binding and production of specific chitosan oligomers.

The value of protein engineering as a tool for the more efficient production of pure oligomers and potentially bioactive paCOS mixtures was demonstrated by the results and the suitability of specific muteins for the large-scale production of strictly defined, pure paCOS in a batch process was shown using the example of D4.