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RECCIPE: A new framework assessing localized cell-cell interaction on gene expression in multicellular ST data.

Weiping MaXiaoyu SongGuo-Cheng YuanPei Wang
Published in: Frontiers in genetics (2024)
Cell-cell interaction (CCI) plays a pivotal role in cellular communication within the tissue microenvironment. The recent development of spatial transcriptomics (ST) technology and associated data analysis methods has empowered researchers to systematically investigate CCI. However, existing methods are tailored to single-cell resolution datasets, whereas the majority of ST platforms lack such resolution. Additionally, the detection of CCI through association screening based on ST data, which has complicated dependence structure, necessitates proper control of false discovery rates due to the multiple hypothesis testing issue in high dimensional spaces. To address these challenges, we introduce RECCIPE, a novel method designed for identifying cell signaling interactions across multiple cell types in spatial transcriptomic data. RECCIPE integrates gene expression data, spatial information and cell type composition in a multivariate regression framework, enabling genome-wide screening for changes in gene expression levels attributed to CCIs. We show that RECCIPE not only achieves high accuracy in simulated datasets but also provides new biological insights from real data obtained from a mouse model of Alzheimer's disease (AD). Overall, our framework provides a useful tool for studying impact of cell-cell interactions on gene expression in multicellular systems.
Keyphrases
  • single cell
  • gene expression
  • rna seq
  • cell therapy
  • data analysis
  • dna methylation
  • mouse model
  • genome wide
  • big data
  • high throughput
  • electronic health record
  • cognitive decline