Activities of a broad-spectrum antimicrobial peptide analogue SAMP-A4-C8 and its combat against pneumonia in Staphylococcus aureus-infected mice.
Rui Fang LiPu HaoKedong YinQingpeng XuShiming RenYingyuan ZhaoLan ZhangBeibei ZhangPublished in: Journal of peptide science : an official publication of the European Peptide Society (2023)
Antimicrobial peptides and their analogues have become substitutes for antibiotics in recent years. The antimicrobial peptide analogue SAMP-A4-C8 (n-octanoic-VRLLRRRI) with high antimicrobial activity was found in our lab. We speculate that it may kill pathogens by some lethal mechanism of action. In the present investigation, the microbicidal activities of SAMP-A4-C8 and its mechanism of action were investigated. The results demonstrated that SAMP-A4-C8 had lethal activities against Staphylococcus aureus and Candida albicans by cell disruption. Based on its microbicidal activities, we believe that it is worth further research for its potential as drug candidate. The results showed that SAMP-A4-C8, with low propensity to induce the resistance of S. aureus and C. albicans, could kill the persister cells of S. aureus and C. albicans, exhibited biofilm forming inhibition activity and preformed biofilm eradication ability against S. aureus and C. albicans, and displayed therapeutic potential on pneumonia in S. aureus-infected mice by reducing lung inflammation. The present study provided a promising drug candidate in the war against multidrug resistance.
Keyphrases
- candida albicans
- biofilm formation
- staphylococcus aureus
- high fat diet induced
- induced apoptosis
- single cell
- pseudomonas aeruginosa
- molecular docking
- metabolic syndrome
- signaling pathway
- insulin resistance
- multidrug resistant
- skeletal muscle
- emergency department
- type diabetes
- helicobacter pylori
- antimicrobial resistance
- mesenchymal stem cells
- bone marrow
- wild type
- helicobacter pylori infection
- cell death
- drug induced