Induction of reproductive activity and egg production by gonadotropin-releasing hormone in non-laying hens.

The current study was conducted to evaluate the effects of gonadotropin-releasing hormone (GnRH) analogue (Receptal) injection on reproductive traits of fully mature layers hens (32 weeks) suffered from inactive ovaries. Ninety-six non-laying hens (TETRA-SL brown egg layers), selected from a commercial flock, with similar body weight, were randomly assigned to four groups (n = 24). Hens in the 1st group served as a control. Hens of the 2nd, 3rd and 4th groups were individually intramuscularly injected every 4 days with 50, 100 and 150 µl of Receptal solution, respectively, for two times. The results stated that the injection of Receptal induced the non-laying hens to produce eggs, but control birds did not produce eggs during the experimental period. The distance between pelvic bones and between the pelvic bone and keel bone of hens was significantly improved (p < .001) in groups received different GnRH levels compared with the control group. The best results were observed in the group injected with 100 µl Receptal. Levels of LH, FSH, oestrogen and progesterone hormones were significantly (p < .05) higher in Receptal-treated groups than in the control group. Hens injected with Receptal had an increase in ovary%, yellow follicles number, oviduct% and oviduct length (p < .001) compared with the control. It was concluded that treating inactive ovaries in non-laying hens with GnRH injections for two times, 4-day intervals, is an effective procedure for inducing egg production and useful in cost reduction in layer farms, and the group treated with 100 µl Receptal had the best results.