Platinum-Selenopeptide Interfaces in Support of High Fidelity Electrochemical Biomarker Quantification in Complex Biological Matrices.
Rui HanYang LiWenqing WangCai-Feng DingJason J DavisXi-Liang LuoPublished in: Analytical chemistry (2024)
The real world applications of conventional antifouling biosensors based on gold-thiol (Au-S) interfaces are hampered by the progressive competitive displacement of key functionality by ubiquitous biothiols. To overcome this limitation, we introduce here novel platinum-selenium (Pt-Se) interfaces. Thiol displacement tests, antifouling analyses, and density functional theory calculations confirm markedly improved interfacial stability. This was then leveraged through the application of a seleno-multifunctional peptide platform, tailored to the detection of murine double minute 2, in biological samples. A derived amperometric sensing platform exhibited a notably lower detection limit and more accurate target quantification than that supported by analogous Au-S and Pt-S interfaces. We believe that this work broadens the scope of electrochemical sensor construction and holds significant promise for the development of high-fidelity impactful bioassay platforms.
Keyphrases
- density functional theory
- label free
- molecular dynamics
- ionic liquid
- gold nanoparticles
- reduced graphene oxide
- loop mediated isothermal amplification
- sensitive detection
- molecular dynamics simulations
- high throughput
- multiple sclerosis
- molecularly imprinted
- drug delivery
- high resolution
- hydrogen peroxide
- big data
- cancer therapy
- fluorescent probe
- artificial intelligence