Activity-based protein profiling reveals dynamic substrate-specific cellulase secretion by saprotrophic basidiomycetes.

Nicholas G S McGregorCasper de BoerMikhaaeel SantosMireille HaonDavid NavarroSybrin SchroderJean-Guy BerrinHerman S OverkleeftGideon J Davies

Published in: Biotechnology for biofuels and bioproducts (2022)

Using small-volume samples with minimal sample preparation, the results presented here demonstrate the ready accessibility of sensitive direct evidence for fungal enzyme secretion during early stages of growth on complex lignocellulosic substrates.

Keyphrases

amino acid
single cell
protein protein
anaerobic digestion
molecularly imprinted
binding protein
small molecule
mass spectrometry
solid phase extraction