In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-1.
Vanessa Araújo VarelaLetícia Borges da Silva HeinenLuciana Cavalheiro MartiVictória Bulcão CaracioloTarcila Santos DatoguiaMariane Tami AmanoWelbert Oliveira PereiraPublished in: Journal of immunological methods (2023)
We developed an effective protocol for MDSC in vitro production from the differentiation of the immature myeloid cell line THP-1 induced by G-CSF and IL-4. Furthermore, we demonstrated that THP1-MDSC-like suppressor cells contribute to the immune escape of AML cells. Potentially, these THP1-MDSC-like cells can be applied on a large-scale platform, thus being able to impact the course of several studies and models such as cancer, immunodeficiencies, autoimmunity, and chronic inflammation.