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Collecting and Culturing Bryozoans for Regenerative Studies.

Abigail M SmithPeter B BatsonKaterina AchilleosYuta Tamberg
Published in: Methods in molecular biology (Clifton, N.J.) (2022)
Among marine invertebrates, bryozoans are small, not well known, and complex to identify. Nevertheless, they offer unique opportunities for whole-body generation research, because of their colonial, modular mode of growth. Here, we describe detailed methods for collection of bryozoans from a range of environments, sample preparation and identification, culture and feeding, spawning and breeding, marking colonies for growth studies, and histological preparation.
Keyphrases
  • stem cells
  • case control
  • molecularly imprinted
  • mesenchymal stem cells
  • cell therapy
  • simultaneous determination