Comprehensive stability-indicating high-performance liquid chromatography coupled with diode array detection method for simultaneous determination of amprolium hydrochloride and ethopabate in powder dosage form for veterinary use.
Mostafa M BakerDina S El-KafrawyMagdi M Abdel-KhalekTarek S BelalPublished in: Journal of separation science (2019)
This research deals with the development of a stability-indicating high-performance liquid chromatography method for simultaneous determination of amprolium hydrochloride and ethopabate. To the best of our knowledge, no comprehensive stability-indicating method has been reported for analysis of this mixture. Separation was achieved using Kromasil cyano column with gradient elution of the mobile phase composed of sodium hexane sulfonate solution and methanol. Quantification was based on measuring peak areas at 266 nm. Amprolium and ethopabate peaks eluted at retention times 10.42 and 18.53 min, respectively. The proposed procedure was validated with respect to system suitability, linearity, ranges, precision, accuracy, specificity, robustness, detection, and quantification limits. Linearity ranges for amprolium and ethopabate were 1.5-240 and 1-160 μg/mL, respectively. Analytes were subjected to stress conditions of hydrolysis, oxidation and thermal degradation. The proposed method enabled resolution of drugs from their forced-degradation products and amprolium related substance (2-picoline). Moreover, specificity was verified by resolution of the analytes from about 22 drugs used in antimicrobial veterinary products. The validated method was successfully applied to assay of the combined veterinary powder dosage form, additionally it was implemented in the accelerated stability study of the dosage form when stored for six months at 40°C and 75% relative humidity.
Keyphrases
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- solid phase extraction
- liquid chromatography
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- ultra high performance liquid chromatography
- gas chromatography
- ms ms
- high resolution mass spectrometry
- high throughput
- hydrogen peroxide
- staphylococcus aureus
- high resolution
- single cell
- heat stress
- drug induced
- single molecule
- nitric oxide
- real time pcr
- minimally invasive