Two new lindenane-type sesquiterpenoid dimers, chlotrichenes C and D (1 and 2) together with five known lindenane-type sesquiterpenoid dimers (3-7) were isolated from the roots of Chloranthus holostegius var. trichoneurus, a famous natural medicine named as "Sikuaiwa" for subduing swellings and relieving pain. The structures including absolute configuration were elucidated by their 1D and 2D NMR, HRESIMS, and ECD data. Compounds 1 and 2 were classical [4 + 2] lindenane-type sesquiterpenoid dimers that differed from known analogs in oxidation profile, side chain profile, and double bond position. The new isolates and compound 3 exhibited significant inhibitory activity on IL-1β production (IC 50 : 1-15 μM) in LPS-induced THP-1 cells and other compounds exhibited inhibitory activity on NO production in LPS-induced RAW 264.7 cells (IC 50 : 24-33 μM).
Keyphrases
- lps induced
- inflammatory response
- induced apoptosis
- cell cycle arrest
- anti inflammatory
- high resolution
- chronic pain
- magnetic resonance
- machine learning
- neuropathic pain
- pain management
- cell death
- oxidative stress
- electronic health record
- molecular docking
- big data
- signaling pathway
- spinal cord injury
- mass spectrometry
- nitric oxide
- pi k akt
- deep learning
- artificial intelligence