Signal-on fluorescence assay for pyrophosphate ions based on DNA-stabilized silver nanoclusters.
Huifeng XuLishuang YuShiqi ZhangXueya XuTingting ChenHongzhi YeXi ZhuPublished in: Luminescence : the journal of biological and chemical luminescence (2019)
Pyrophosphate anion (P2 O7 4- , PPi) is considered as a potential biomarker for arthritic diseases because high levels of PPi may result in calcium pyrophosphate dehydrate crystal deposition diseases. In this study, a simple fluorescence method for PPi was demonstrated by organic integration of the efficient fluorescence quenching ability of copper ions to DNA-scaffolded silver nanoclusters and the strong affinity of PPi towards copper ions. This simple fluorescence sensor showed a low detection limit (0.28 μM based on signal/noise = 3) towards the detection of PPi. Practical application of this method was also validated by detection of PPi in the synovial fluid.
Keyphrases
- energy transfer
- single molecule
- protein protein
- label free
- quantum dots
- loop mediated isothermal amplification
- small molecule
- sensitive detection
- gold nanoparticles
- real time pcr
- circulating tumor
- water soluble
- cell free
- air pollution
- aqueous solution
- high throughput
- silver nanoparticles
- oxide nanoparticles
- circulating tumor cells
- capillary electrophoresis