Ultrananocrystalline diamond-coated nanoporous membranes support SK-N-SH neuroblastoma epithelial [corrected] cell attachment.
Kai-Hung YangAlexander K NguyenPeter L GoeringAnirudha V SumantRoger J NarayanPublished in: Interface focus (2018)
Ultrananocrystalline diamond (UNCD) has been demonstrated to have attractive features for biomedical applications and can be combined with nanoporous membranes for applications in drug delivery systems, biosensing, immunoisolation and single molecule analysis. In this study, free-standing nanoporous UNCD membranes with pore sizes of 100 or 400 nm were fabricated by directly depositing ultrathin UNCD films on nanoporous silicon nitride membranes and then etching away silicon nitride using reactive ion etching. Successful deposition of UNCD on the substrate with a novel process was confirmed with Raman spectroscopy, X-ray photoelectron spectroscopy, cross-section scanning electron microscopy (SEM) and transmission electron microscopy. Both sample types exhibited uniform geometry and maintained a clear hexagonal pore arrangement. Cellular attachment of SK-N-SH neuroblastoma endothelial cells was examined using confocal microscopy and SEM. Attachment of SK-N-SH cells onto UNCD membranes on both porous regions and solid surfaces was shown, indicating the potential use of UNCD membranes in biomedical applications such as biosensors and tissue engineering scaffolds.
Keyphrases
- electron microscopy
- single molecule
- tissue engineering
- metal organic framework
- raman spectroscopy
- endothelial cells
- high resolution
- induced apoptosis
- quantum dots
- risk assessment
- cell cycle arrest
- cell death
- mesenchymal stem cells
- living cells
- gold nanoparticles
- pseudomonas aeruginosa
- cystic fibrosis
- escherichia coli
- vascular endothelial growth factor
- climate change
- endoplasmic reticulum stress
- high glucose
- pi k akt
- light emitting