Substituted meso-vinyl-BODIPY as thiol-selective fluorogenic probes for sensing unfolded proteins in the endoplasmic reticulum.

Huiying MuKoji MikiTakuya Kubo Koji Otsuka Kouichi Ohe

Published in: Chemical communications (Cambridge, England) (2021)

A new type of thiol probes based on the meso-vinyl-BODIPY (VB) scaffold were developed. The monochloro-substituted VB1Cl exhibited the largest fluorescence enhancement (>200-fold) as well as high selectivity upon biological thiol sensing. VB1Cl was successfully applied for reporting the protein unfolding process under ER stress in living cells.

Keyphrases

living cells
endoplasmic reticulum
fluorescent probe
single molecule
molecular docking
adverse drug
endoplasmic reticulum stress
binding protein
quantum dots