The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks.
Ramsés A Gamboa-SuasnavartNorma A Valdez-CruzGerardo Gaytan-OrtegaGreta I Reynoso-CerecedaDaniel Cabrera-SantosLorena López-GriegoWolf KlöcknerJochen BüchsMauricio A Trujillo-RoldánPublished in: Microbial cell factories (2018)
Although the metabolic switch is usually related to limitations in phosphate and nitrogen in Streptomyces sp., our results reveal that it can also be activated by low OTR, dramatically affecting recombinant glycoprotein production and O-mannosylation and increasing RED synthesis in the process.