Tetraacetyl Riboflavin Derivative Mediates Caspase 3/7 Activation via MAPK in A431 Cells upon Blue Light Influence.
Izabela BuniowskaNorbert WronskiMałgorzata Insinska-RakMarek SikorskiAgnieszka Wolnicka-GlubiszPublished in: Photochemistry and photobiology (2023)
An acetylated riboflavin derivative, 3-methyl-tetraacetyl riboflavin (3MeTARF), is a compound with high photostability and photophysical properties similar to riboflavin, including the ability to photogenerate singlet oxygen. In the present study, we compared the effects of irradiation on A431 cancer cells with blue LED light (438 nm) in the presence of 3MeTARF and riboflavin on MAPK phosphorylation, apoptosis, caspase 3/7 activation and PARP cleavage. We observed that photogenerated oxidative stress in this reaction activates MAPK by increasing phosphorylation of p38 and JNK proteins. Preincubation of cells with inhibitors specific for phosphorylation of p38 and JNK proteins (SB203580, SP600125) respectively, results in decreased caspase 3/7 activation and PARP cleavage. We showed that the tetraacetyl derivative more effectively activates MAPK and skin cancer cell death compared to riboflavin. These data, together with results of our previous study, support the hypothesis that 3MeTARF, of riboflavin, might be more useful and desirable as a compound for use in photodynamic oxidation processes, including its therapeutic potential.
Keyphrases
- induced apoptosis
- oxidative stress
- cell death
- signaling pathway
- cell cycle arrest
- endoplasmic reticulum stress
- pi k akt
- dna damage
- ischemia reperfusion injury
- diabetic rats
- radiation therapy
- skin cancer
- dna binding
- dna repair
- nitric oxide
- photodynamic therapy
- hydrogen peroxide
- transcription factor
- cancer therapy
- deep learning
- radiation induced
- drug delivery
- water soluble