Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines in Streptomyces sp. XS-16.
Xiao XuFa-Lei ZhangLuning ZhouYimin ChangQian CheTianjiao ZhuDe-Hai LiGuojian ZhangPublished in: Marine drugs (2023)
Six angucyclines including three unreported compounds ( 1 - 3 ) were isolated from Streptomyces sp. XS-16 by overexpressing the native global regulator of SCrp (cyclic AMP receptor). The structures were characterized based on nuclear magnetic resonance (NMR) and spectrometry analysis and assisted by electronic circular dichroism (ECD) calculations. All compounds were tested for their antitumor and antimicrobial activities, and compound 1 showed different inhibitory activities against various tumor cell lines with IC 50 values ranging from 0.32 to 5.33 μM.
Keyphrases
- magnetic resonance
- high resolution
- transcription factor
- small molecule
- staphylococcus aureus
- cell proliferation
- contrast enhanced
- high throughput
- magnetic resonance imaging
- density functional theory
- protein kinase
- molecular dynamics
- molecular dynamics simulations
- mass spectrometry
- liquid chromatography
- binding protein
- simultaneous determination
- solid phase extraction