Refactoring of a synthetic raspberry ketone pathway with EcoFlex.
Simon J MooreYonek B HlebaSarah BischoffDavid BellKaren M PolizziPaul S FreemontPublished in: Microbial cell factories (2021)
Our findings provide a stable raspberry ketone pathway that relies upon a natural feedstock (L-tyrosine) and uses only constitutive promoters to control gene expression. In conclusion we demonstrate the capability of EcoFlex for fine-tuning a model fine chemical pathway and provide a range of newly characterised promoter tools gene expression in E. coli.