Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.
Kaylee M ClarkMelissa S SchenkelTrey W PittmanIsabelle C SamperLoran B R AndersonWisarut KhamcharoenSuad ElmegerhiRushika PereraWeena SiangprohAlan J KennanBrian J GeissDavid S DandyCharles S HenryPublished in: ACS measurement science au (2022)
The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich immunoassay with a single end-user step. The modified electrodes are sensitive and selective for SARS-CoV-2 N protein and stable for over 7 weeks. The eCaDI was tested with influenza A and Sindbis virus and proved to be selective. The eCaDI was also successfully applied to detect nine different SARS-CoV-2 variants, including Omicron.
Keyphrases
- sars cov
- low cost
- label free
- respiratory syndrome coronavirus
- gold nanoparticles
- sensitive detection
- carbon nanotubes
- loop mediated isothermal amplification
- protein protein
- high resolution
- working memory
- ionic liquid
- molecularly imprinted
- real time pcr
- high throughput
- amino acid
- copy number
- pet ct
- coronavirus disease
- room temperature
- reduced graphene oxide
- single cell
- electron transfer