Loop-mediated Isothermal Amplification and nested PCR of the Internal Transcribed Spacer (ITS) for Histoplasma capsulatum detection.
Matheus da Silva ZattiThales Domingos ArantesJosé Alex Lourenço FernandesMônica Baumgardt BayEveline Pipolo MilanGeorggia Fatima Silva NaliatoRaquel Cordeiro TheodoroPublished in: PLoS neglected tropical diseases (2019)
ITS LAMP and nPCR assays have the potential to be used in conjunction with culture for early diagnosis of progressive disseminated histoplasmosis, allowing earlier, appropriate treatment of the patient. The possibility of applying ITS LAMP, as a direct assay, with no DNA extraction and purification steps, makes it suitable for resource-limited laboratories. However, more studies are necessary to validate ITS LAMP and nPCR as direct assay in other types of clinical specimens.