Characterization of the development of the mouse cochlear epithelium at the single cell level.
Likhitha KollaMichael C KellyZoë F MannAlejandro Anaya-RochaKathryn EllisAbigail LemonsAdam T PalermoKathy S SoJoseph C MaysJoshua OrvisJoseph C BurnsRonna HertzanoElizabeth Carroll DriverMatthew William KelleyPublished in: Nature communications (2020)
Mammalian hearing requires the development of the organ of Corti, a sensory epithelium comprising unique cell types. The limited number of each of these cell types, combined with their close proximity, has prevented characterization of individual cell types and/or their developmental progression. To examine cochlear development more closely, we transcriptionally profile approximately 30,000 isolated mouse cochlear cells collected at four developmental time points. Here we report on the analysis of those cells including the identification of both known and unknown cell types. Trajectory analysis for OHCs indicates four phases of gene expression while fate mapping of progenitor cells suggests that OHCs and their surrounding supporting cells arise from a distinct (lateral) progenitor pool. Tgfβr1 is identified as being expressed in lateral progenitor cells and a Tgfβr1 antagonist inhibits OHC development. These results provide insights regarding cochlear development and demonstrate the potential value and application of this data set.
Keyphrases
- single cell
- induced apoptosis
- gene expression
- cell therapy
- cell cycle arrest
- rna seq
- hearing loss
- minimally invasive
- oxidative stress
- cell death
- transforming growth factor
- machine learning
- electronic health record
- risk assessment
- epithelial mesenchymal transition
- bone marrow
- artificial intelligence
- deep learning
- cell fate