Measuring fucosylated alpha-fetoprotein in hepatocellular carcinoma: A comparison of μTAS and parallel reaction monitoring.
Kwang Hoe KimSang Yoon LeeJe-Hyun BaekSoo-Youn LeeJin Young KimJong Shin YooPublished in: Proteomics. Clinical applications (2021)
Fucosylation of AFP is used for the detection of HCC. A micro-total analysis system (μTAS) has been only developed for measuring fucosylation of AFP in clinical research. This study reports the fucosylation of AFP in human serum samples from cirrhosis and HCC patients using the μTAS and a LC-PRM MS to evaluate fucosylation of AFP from each method. As a result, LC-PRM MS is complementary to the conventional μTAS method. Furthermore, LC-PRM MS provides a higher diagnostic accuracy than the μTAS in patients with low AFP levels and an early stage.
Keyphrases
- mass spectrometry
- early stage
- multiple sclerosis
- ms ms
- simultaneous determination
- liquid chromatography
- end stage renal disease
- ejection fraction
- prognostic factors
- peritoneal dialysis
- solid phase extraction
- squamous cell carcinoma
- patient reported outcomes
- tandem mass spectrometry
- high resolution mass spectrometry
- loop mediated isothermal amplification
- neoadjuvant chemotherapy
- data analysis