Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite.
S MeyerA López-SerranoH MitzeN JakubowskiTanja SchwerdtlePublished in: Metallomics : integrated biometal science (2019)
Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.
Keyphrases
- single cell
- ms ms
- rna seq
- mass spectrometry
- high throughput
- liquid chromatography tandem mass spectrometry
- minimally invasive
- high performance liquid chromatography
- cell therapy
- induced apoptosis
- stem cells
- liquid chromatography
- endoplasmic reticulum stress
- cell cycle arrest
- case control
- sensitive detection
- gas chromatography
- ultra high performance liquid chromatography
- real time pcr