A highly sensitive signal-on biosensor for microRNA 142-3p based on the quenching of Ru(bpy)32+-TPA electrochemiluminescence by carbon dots and duplex specific nuclease-assisted target recycling amplification.

Jiao YangQian XiaLonghua GuoFang Luo Yongqiang Dong Bin Qiu Zhenyu Lin

Published in: Chemical communications (Cambridge, England) (2021)

Carbon dots (CDs) have been used to quench the electrochemiluminescence (ECL) of a Ru(bpy)32+-tripropylamine (TPA) system for the first time. In addition, this phenomenon has been coupled with duplex specific nuclease-assisted target recycling amplification to develop a highly sensitive ECL biosensor for microRNA.

Keyphrases

energy transfer
quantum dots
label free
sensitive detection
nucleic acid
gold nanoparticles
dna binding
fluorescent probe
molecularly imprinted
transcription factor