Targeted Delivery of Anticancer Drug Loaded Charged PLGA Polymeric Nanoparticles Using Electrostatic Field.

Pure Positive electrostatic charges (PPECs) showed the suppressive effect on the proliferation and metabolism of invasive cancer cells without affecting normal tissues. PPECs were used for the delivery of drug-loaded (Paclitaxel) polymeric nanoparticles (DLNs) capped with negatively charged poly(lactide-coglycolide) (PLGA) and Poly(vinyl-alcohol) PVA into the tumor site of mouse models. The charged patch was installed on top of the skin in the mouse models' tumor region, and the controlled selective release of the drug was assayed by biochemical, radiological, and histological experiments on both tumorized models and normal rats' livers. We found that DLNs synthesized by PLGA show great attraction to PPECs due to their stable negative charges, which would not degrade immediately in the blood. The burst and drug release after less than 48 hours of our synthesized DLNs were 10% and 50%, respectively. These compounds can deliver the loaded-drug into the tumor site with the assistance of PPECs, and the targeted-retarded release will take place. Hence, local therapy could be achieved with a much lower drug concentration (conventional chemotherapy [2 mg.kg-1] versus DLNs-based chemotherapy [0.75 mg.kg-1]) with negligible side effects in non-targeted organs. PPECs have many potential clinical applications for advanced-targeted chemotherapy with the lowest discernible side effects. This article is protected by copyright. All rights reserved.