A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.
Inwha BaekSarah N LeJongcheol JeonYujin ChunCharlotte ReedStephen BuratowskiPublished in: G3 (Bethesda, Md.) (2022)
Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed.
Keyphrases
- saccharomyces cerevisiae
- small molecule
- protein protein
- fluorescence imaging
- quantum dots
- living cells
- amino acid
- binding protein
- photodynamic therapy
- aqueous solution
- gene therapy
- computed tomography
- wastewater treatment
- label free
- pet imaging
- single molecule
- positron emission tomography
- endoscopic submucosal dissection