Optical Cell Tagging for Spatially Resolved Single-Cell RNA Sequencing.
Qi TangLu LiuYilan GuoXu ZhangShaoran ZhangYan JiaYifei DuBo ChengLi YangYanyi HuangXing ChenPublished in: Angewandte Chemie (International ed. in English) (2021)
Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for profiling gene expression of distinct cell populations at the single-cell level. However, the information of the positions of cells within the multicellular samples is missing in scRNA-seq datasets. To overcome this limitation, we herein develop OpTAG (optical cell tagging) as a new chemical platform for attaching functional tags onto cell surfaces in a spatially resolved manner. With OpTAG, we establish OpTAG-seq, which enables spatially resolved scRNA-seq. We apply OpTAG-seq to investigate the spatially defined transcriptional program in migrating cancer cells and identified a list of genes that are potential regulators for cancer cell migration and invasion. OpTAG-seq provides a convenient method for mapping cellular heterogeneity with spatial information within multicellular biological systems.
Keyphrases
- single cell
- rna seq
- high throughput
- gene expression
- high resolution
- dna methylation
- escherichia coli
- transcription factor
- healthcare
- cystic fibrosis
- stem cells
- risk assessment
- oxidative stress
- cell death
- health information
- staphylococcus aureus
- heat shock
- endoplasmic reticulum stress
- high density
- human health
- biofilm formation
- genetic diversity
- genome wide identification
- pi k akt