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Full-field optical coherence tomography: novel imaging technique for extemporaneous high-resolution analysis of mucosal architecture in human gut biopsies.

Lucille QuénéhervéRaphael OlivierMichalina J GoraCéline BossardJean-François MosnierEmilie Benoit A la GuillaumeClaude BoccaraCharlène BrochardMichel NeunlistEmmanuel Coron
Published in: Gut (2020)
Full-field optical coherence tomography (FFOCT) is an imaging technique of biological tissue based on tissue light reflectance analysis. We evaluated the feasibility of imaging fresh digestive mucosal biopsies after a quick mounting procedure (5 min) using two distinct modalities of FFOCT. In static FFOCT mode, we gained high-resolution images of general gut tissue-specific architecture, such as oesophageal papillae, gastric pits, duodenal villi and colonic crypts. In dynamic FFOCT mode, we imaged individual epithelial cells of the mucosal lining with a cellular or subcellular resolution and identified cellular components of the lamina propria. FFOCT represents a promising dye-free imaging tool for on-site analysis of gut tissue remodelling.
Keyphrases
  • high resolution
  • optical coherence tomography
  • mass spectrometry
  • ulcerative colitis
  • endothelial cells
  • deep learning
  • diabetic retinopathy
  • machine learning
  • high speed
  • single molecule
  • liquid chromatography