Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols.
Omri ShelefAdam C SedgwickSabina PozziOri GreenRonit Satchi-FainaroDoron ShabatJonathan L SesslerPublished in: Chemical communications (Cambridge, England) (2021)
We report a chemiluminescent probe (CLPT1) that permits the paired detection of tyrosinase (Tyr) and biological thiols. Tyr only leads to a poor chemiluminescence response, a finding ascribed to the formation of a stable o-benzoquinone intermediate. The addition of glutathione (GSH), or ascorbate to the o-benzoquinone intermediate results in thiol conjugation or reduction to this intermediate, respectively. This produces a strong chemiluminescence response. Thiol co-dependence was demonstrated in live cells using the cell permeable analogue, CLPT3. The present chemiluminescence-based strategy allows the concurrent detection of tyrosinase activity and biological thiols.
Keyphrases
- sensitive detection
- loop mediated isothermal amplification
- living cells
- quantum dots
- energy transfer
- molecularly imprinted
- fluorescent probe
- induced apoptosis
- real time pcr
- label free
- single cell
- cell therapy
- cell cycle arrest
- radiation therapy
- oxidative stress
- cell death
- fluorescence imaging
- bone marrow
- endoplasmic reticulum stress
- signaling pathway
- squamous cell carcinoma
- rectal cancer
- photodynamic therapy
- high resolution
- simultaneous determination
- nucleic acid
- tandem mass spectrometry