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Evolutionary Ancestry of Eukaryotic Protein Kinases and Choline Kinases.

Shenshen LaiJavad SafaeiSteven Pelech
Published in: The Journal of biological chemistry (2016)
The reversible phosphorylation of proteins catalyzed by protein kinases in eukaryotes supports an important role for eukaryotic protein kinases (ePKs) in the emergence of nucleated cells in the third superkingdom of life. Choline kinases (ChKs) could also be critical in the early evolution of eukaryotes, because of their function in the biosynthesis of phosphatidylcholine, which is unique to eukaryotic membranes. However, the genomic origins of ePKs and ChKs are unclear. The high degeneracy of protein sequences and broad expansion of ePK families have made this fundamental question difficult to answer. In this study, we identified two class-I aminoacyl-tRNA synthetases with high similarities to consensus amino acid sequences of human protein-serine/threonine kinases. Comparisons of primary and tertiary structures supported that ePKs and ChKs evolved from a common ancestor related to glutaminyl aminoacyl-tRNA synthetases, which may have been one of the key factors in the successful of emergence of ancient eukaryotic cells from bacterial colonies.
Keyphrases
  • amino acid
  • protein protein
  • binding protein
  • endothelial cells
  • oxidative stress
  • genome wide
  • cell death
  • dna methylation
  • small molecule
  • mass spectrometry
  • copy number
  • induced pluripotent stem cells