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New Cell Lines Derived from Laboratory Colony Triatoma infestans and Rhodnius prolixus , Vectors of Trypanosoma cruzi , Do Not Harbour Triatoma Virus.

Rebekah Penrice-RandalCatherine HartleyAlexandra BeliavskaiaXiaofeng DongLuke Brandner-GarrodMiranda WhittenLesley Bell-Sakyi
Published in: Insects (2022)
Triatomine bugs of the genera Triatoma and Rhodnius are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by Trypanosoma cruzi . Triatoma virus (TrV), a natural pathogen of Triatoma infestans , has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for in vitro propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of T. infestans (TIE/LULS54) and Rhodnius prolixus (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect R. prolixus , indicating that these new cell lines could be used for propagation of TrV.
Keyphrases
  • trypanosoma cruzi
  • induced apoptosis
  • climate change
  • cell cycle arrest
  • gene therapy
  • candida albicans
  • oxidative stress
  • signaling pathway
  • cell proliferation