Impact of cfDNA Reference Materials on Clinical Performance of Liquid Biopsy NGS Assays.

Ariane HallermayrThomas KeßlerMoritz FujeraBen LiesfeldSamuel BernsteinSimon von AmelnDenny SchanzeVerena Steinke-LangeJulia M A PicklTeresa M NeuhannElke Holinski-Feder

Published in: Cancers (2023)

Careful consideration of commercial reference materials is required for performance evaluation of liquid biopsy NGS assays. While the similarity to native cfDNA aids in the development of experimental protocols, reference materials with well-defined variants are preferable for determining sensitivity and precision, which are essential for accurate clinical interpretation.

Keyphrases

high throughput
ultrasound guided
ionic liquid
copy number
gene expression
antiretroviral therapy
mass spectrometry
genome wide