Multifunctional Fe3O4@Polydopamine@DNA-Fueled Molecular Machine for Magnetically Targeted Intracellular Zn2+ Imaging and Fluorescence/MRI Guided Photodynamic-Photothermal Therapy.
Yao YaoDan ZhaoNa LiFuzhi ShenJeremiah Ong'achwa MachukiDongzhi YangJingjing LiDaoquan TangYanyan YuJiangwei TianHaifeng DongFenglei GaoPublished in: Analytical chemistry (2019)
For the precise treatment of tumors, it is necessary to develop a theranostic nanoplatform that has both diagnostic and therapeutic functions. In this article, we designed a new theranostic probe for fluorescence imaging of Zn2+ and fluorescence/MRI guided magnetically targeted photodynamic-photothermal therapy. The fluorescence imaging of Zn2+ was based on an endogenous ATP-driven DNA nanomachine that could perform repetitive stand displacement reaction. It modifies all units on a single PDA/Fe3O4 nanoparticle containing a hairpin-locked initiated strand activated by a target molecule in cells, a two-stranded fuel DNA triggered by ATP, and a two-stranded DNA track responding to an initiated strand and fuel DNA. After entering the cell, the intracellular target Zn2+ initiates the nanomachine via an autocatalytic cleavage reaction, and the machine programmatically and gradually runs on the assembled DNA track via fuel DNA driving and the intramolecular toehold-mediated stand displacement reaction. The Fe3O4 core first exhibits magnetic targeting, increasing the ability of nanoparticles to enter tumor cells at the tumor site. The Fe3O4 could also be employed as a powerful magnetic resonance imaging (MRI) contrast agent and guided therapy. Using 808 nm laser and 635 nm laser irradiation together at the tumor site, the PDA nanoshell produced an excellent photothermal effect and the TMPyP4 molecules entering the cell generated reactive oxygen species, followed by cell damage. A series of reliable experiments suggested that the Fe3O4@PDA@DNA nanoprobe showed superior fluorescence specificity, MRI, a remarkable photothermal/photodynamic therapy effect, and favorable biocompatibility. This theranostic nanoplatform offered a split-new insight into tumor fluorescence and MRI diagnosis as well as effective tumor therapy.
Keyphrases
- photodynamic therapy
- fluorescence imaging
- single molecule
- circulating tumor
- cancer therapy
- magnetic resonance imaging
- cell free
- contrast enhanced
- nucleic acid
- drug delivery
- reactive oxygen species
- living cells
- heavy metals
- single cell
- computed tomography
- diffusion weighted imaging
- cell therapy
- energy transfer
- high frequency
- magnetic resonance
- induced apoptosis
- machine learning
- deep learning
- bone marrow
- transcription factor
- cell proliferation
- signaling pathway
- endoplasmic reticulum stress
- molecularly imprinted
- cell cycle arrest
- dna binding