Application of Hydrogen/Deuterium Exchange-Mass Spectrometry to Biopharmaceutical Development Requirements: Improved Sensitivity to Detection of Conformational Changes.
Lea BonningtonIngo LindnerUlrich GillesTobias KailichDietmar ReuschPatrick BulauPublished in: Analytical chemistry (2017)
The usefulness of the higher-order structure information provided by hydrogen/deuterium exchange mass spectrometry (HDX-MS) in the protein therapeutic field is undisputed; however, its applicability as a method for critical quality and comparability assessment has until now not been demonstrated. Here we present results demonstrating for the first time the applicability of the HDX-MS technique to monitor structural changes due to methionine oxidation at sensitivity levels realistic to the requirements of biopharmaceutical research and development. For the analyzed heavy chain marker peptides deuterium uptake differences due to oxidation at the conserved methionine in position 254 were significantly verifiable at the lowest increase (1%) through spiked oxidized IgG1.
Keyphrases
- mass spectrometry
- liquid chromatography
- amino acid
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- high resolution
- hydrogen peroxide
- multiple sclerosis
- visible light
- ms ms
- tandem mass spectrometry
- protein protein
- loop mediated isothermal amplification
- social media
- quantum dots
- sensitive detection