Multifunctional nanotherapeutics for intracellular trafficking of doxorubicin against breast cancer.
Balak Das KurmiPreeti PatelRishi PaliwalPramod KumarShivani Rai PaliwalPublished in: Nanomedicine (London, England) (2023)
Aims: To develop an estrone-targeted d-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS)-based liposomal system for enhanced intracellular delivery of doxorubicin (DOX). Materials & methods: Zetasizer, transmission electron microscopy, energy dispersive x-ray, Fourier-transform infrared spectroscopy, differential scanning calorimetry, x-ray diffraction, confocal laser scanning microscopy and FACS analysis were used for formulation characterization and evaluation. Results: The DOX-LIPO-TPGS and DOX-LIPO-TPGS-estrone formulations had vesicle sizes (117.6 ± 3.51; 144 ± 5.00 nm), zeta potential (-36.4 ± 0.75; -35.8 ± 0.76), polydispersity index (0.123 ± 0.005; 0.169 ± 0.005) and percent entrapment efficiency (73.56 ± 3.55; 77.16 ± 3.83%) with improved cytotoxicity and cellular uptake, confirming the targeted potential of the developed formulations. Conclusion: The results suggest that the developed liposomal formulation with desired characteristics is potentially capable of nonimmunogenic, site-specific drug delivery to targeted cancer sites and reduced DOX-associated cardiac toxicity.
Keyphrases
- electron microscopy
- drug delivery
- cancer therapy
- high resolution
- drug release
- high speed
- human health
- oxidative stress
- reactive oxygen species
- papillary thyroid
- squamous cell carcinoma
- ionic liquid
- left ventricular
- high throughput
- magnetic resonance
- young adults
- mass spectrometry
- risk assessment
- solid phase extraction
- atrial fibrillation