Securinega Alkaloids from the Twigs of Securinega suffruticosa and Their Biological Activities.
Kyoung Jin ParkChung Sub KimZahra KhanJoonseok OhSun Yeou KimSang Un ChoiKang Ro LeePublished in: Journal of natural products (2019)
Seven new Securinega alkaloids, securingines A-G (1-7), together with seven known analogues (8-14), were isolated from the twigs of Securinega suffruticosa. Their chemical structures were elucidated by a combined approach of spectroscopic analysis, chemical methods, ECD calculations, and DP4+ probability analysis. The full NMR assignments and the absolute configuration of compound 8 are also reported. In addition, all the isolated phytochemicals (1-14) were assessed for their cytotoxic, anti-inflammatory, and potential neuroprotective activities. Compound 4 showed cytotoxic activity (IC50 values of 1.5-6.8 μM) against four human cell lines (A549, SK-OV-3, SK-MEL-2, and HCT15). Compounds 3, 10, 12, and 13 showed potent inhibitory effects on nitric oxide production (IC50 values of 12.6, 12.1, 1.1, and 7.7 μM, respectively) in lipopolysaccharide-stimulated murine microglia BV-2 cells. Compound 5 exhibited a nerve growth factor production effect (172.6 ± 1.2%) in C6 glioma cells at 20 μg/mL.
Keyphrases
- growth factor
- nitric oxide
- anti inflammatory
- inflammatory response
- induced apoptosis
- high resolution
- cell cycle arrest
- magnetic resonance
- lps induced
- toll like receptor
- oxidative stress
- density functional theory
- mass spectrometry
- lipopolysaccharide induced
- molecular dynamics
- brain injury
- climate change
- spinal cord
- subarachnoid hemorrhage
- human health
- spinal cord injury
- immune response
- nitric oxide synthase
- structure activity relationship