Evaluation of the Antiproliferative Potential of Eugenia pyriformis Leaves in Cervical Cancer Cells.
Thiago De Paula AlvesMariana Toledo Martins PereiraThiago Sardou CharretJúlio César Thurler JúniorGuilherme Freimann WermelingerAndrea Regina de Souza BaptistaBruno Kaufmann RobbsAlexandra Christine Helena Frankland SawayaVinicius D'Ávila Bitencourt PascoalA C R F PascoalPublished in: Chemistry & biodiversity (2022)
Eugenia pyriformis, typically known as uvaia, ubaia, uvaieira, uvalha or uvalha-do-campo, is a plant representative of the Myrtaceae family. E. pyriformis decreased HeLa cells proliferation, can induce cell death and reduce cell migration that may be related to metastasis and induction of cell death by apoptosis in vitro assays. Its leaves are used in folk medicine for hypertension control, decreased cholesterol and uric acid, slimming, astringent, and digestive. In this work, the evaluation of the in vitro anticancer potential Cervical Cancer (HeLa cells) and phytochemical analysis in E. pyriformes was performed. It was possible to quantify phenolic compounds and total flavonoids and identify Chlorogenic acid, Quercetrin, and Myricitrin in this species. The crude extract and ethyl acetate fraction inhibited cell viability by 50 % in the dose of 44.42 μg/mL and 40.39 μg/mL, respectively. The induced effector caspase 3/7 activation, which results in apoptosis and the ethyl acetate fraction, decreases cell migration of cancer cell lines; it is responsible for the cleavage of several cellular proteins that will result in the classic phenotype of the apoptotic cell.
Keyphrases
- cell cycle arrest
- cell death
- cell migration
- uric acid
- pi k akt
- blood pressure
- metabolic syndrome
- signaling pathway
- single cell
- oxidative stress
- stem cells
- ionic liquid
- human health
- squamous cell carcinoma
- high glucose
- bone marrow
- young adults
- risk assessment
- lymph node metastasis
- cell proliferation
- immune response
- transcription factor