Identification of Individual Bacterial Cells through the Intermolecular Interactions with Peptide-Functionalized Solid-State Pores.
Makusu TsutsuiMasayoshi TanakaTakahiro MaruiKazumichi YokotaTakeshi YoshidaAkihide ArimaWataru TonomuraMasateru TaniguchiTakashi WashioMina OkochiTomoji KawaiPublished in: Analytical chemistry (2018)
Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLR5-derived recognition peptides and flagella induce ligand-specific perturbations in the translocation dynamics of Escherichia coli, which facilitated the discrimination between the wild-type and flagellin-deletion mutant (ΔfliC) by the resistive pulse patterns thereby demonstrating the sensing of bacteria at a single-cell level. These results provide a novel concept of utilizing weak intermolecular interactions as a recognition probes for single-cell microbial identification.
Keyphrases
- toll like receptor
- wild type
- single cell
- solid state
- inflammatory response
- nuclear factor
- escherichia coli
- rna seq
- immune response
- induced apoptosis
- energy transfer
- high throughput
- blood pressure
- microbial community
- cell cycle arrest
- bioinformatics analysis
- sensitive detection
- oxidative stress
- loop mediated isothermal amplification
- candida albicans
- pseudomonas aeruginosa
- reduced graphene oxide
- mass spectrometry
- high resolution
- amino acid
- pi k akt