In vivo targeting of breast cancer with a vasculature-specific GQDs/hMSN nanoplatform.
Jingjing DongXinyue YaoShian SunYuanyuan ZhongChuntong QianDongzhi YangPublished in: RSC advances (2019)
According to our previous experiment, graphene quantum dots capped in hollow mesoporous silica nanoparticles, denoted as GQDs@hMSN, and its conjugates exhibited great potential for medical applications due to their commendable biocompatibility. Due to the fluorescence and structural stability, and enormous porosity, polyethylene glycol (PEG) modified GQDs@hMSN (GQDs@hMSN-PEG) is a good candidate in a drug carrying and delivery system. However, the goal of targeted drug delivery couldn't be achieved simply by utilizing the enhanced permeability and retention (EPR) effect of tumors. In this study, GQDs@hMSN-PEG was further functionalized with vascular endothelial growth factor antibodies (VEGF Abs) for VEGF targeting of breast tumors. Doxorubicin (DOX) was loaded into GQDs@hMSN-VEGF Abs with a drug loading capacity of 0.80 mg DOX per mg GQDs@hMSN. With GQDs as the fluorescent source, GQDs@hMSN-VEGF Abs demonstrated strong fluorescence intensity in VEGF-positive cells. Results from in vitro and in vivo targeting experiments indicated that GQDs@hMSN-VEGF Abs had high specificity on tumor vasculature, and it could be used as an image-guidable, tumor-selective delivery nanoplatform for breast cancer.
Keyphrases
- vascular endothelial growth factor
- cancer therapy
- drug delivery
- endothelial cells
- quantum dots
- drug release
- photodynamic therapy
- healthcare
- emergency department
- induced apoptosis
- single molecule
- cell proliferation
- deep learning
- signaling pathway
- climate change
- energy transfer
- sensitive detection
- highly efficient
- endoplasmic reticulum stress
- pi k akt
- simultaneous determination
- carbon nanotubes
- high intensity
- cell death
- human health