Blood plasma derived extracellular vesicles (BEVs): particle purification liquid chromatography (PPLC) and proteomic analysis reveals BEVs as a potential minimally invasive tool for predicting response to breast cancer treatment.
Folnetti A AlvarezHussein KaddourYuan LyuChristina PreeceJules CohenLea BaerAlison T StopeckPatricia ThompsonChioma M OkeomaPublished in: Breast cancer research and treatment (2022)
PPLC-based EV isolation provides a preanalytical separation process for BEVs devoid of most contaminants. Our findings suggest that PPLC-isolated BEVs and the five associated proteins may be established as predictors of chemoresistance, and thus serve to identify NR to spare them the toxic effects of NAC.
Keyphrases
- liquid chromatography
- minimally invasive
- mass spectrometry
- high resolution mass spectrometry
- tandem mass spectrometry
- transcription factor
- simultaneous determination
- drinking water
- solid phase extraction
- robot assisted
- label free
- gas chromatography
- recombinant human
- risk assessment
- cancer stem cells
- genome wide analysis