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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy.

Youhua ChenWenjie LiuZhimin ZhangCheng ZhengYujia HuangRuizhi CaoDazhao ZhuLiang XuMeng ZhangYu-Hui ZhangJiannan FanLuhong JinYingke XuCuifang KuangXu Liu
Published in: Nature communications (2018)
Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.
Keyphrases
  • high resolution
  • single molecule
  • cell migration
  • high speed
  • optical coherence tomography
  • high throughput
  • mass spectrometry
  • label free
  • signaling pathway
  • cell proliferation