Insight into plant cell wall chemistry and structure by combination of multiphoton microscopy with Raman imaging.
Zsuzsanna HeinerIngrid ZeiseRivka ElbaumJanina KneippPublished in: Journal of biophotonics (2017)
Spontaneous Raman scattering microspectroscopy, second harmonic generation (SHG) and 2-photon excited fluorescence (2PF) were used in combination to characterize the morphology together with the chemical composition of the cell wall in native plant tissues. As the data obtained with unstained sections of Sorghum bicolor root and leaf tissues illustrate, nonresonant as well as pre-resonant Raman microscopy in combination with hyperspectral analysis reveals details about the distribution and composition of the major cell wall constituents. Multivariate analysis of the Raman data allows separation of different tissue regions, specifically the endodermis, xylem and lumen. The orientation of cellulose microfibrils is obtained from polarization-resolved SHG signals. Furthermore, 2-photon autofluorescence images can be used to image lignification. The combined compositional, morphological and orientational information in the proposed coupling of SHG, Raman imaging and 2PF presents an extension of existing vibrational microspectroscopic imaging and multiphoton microscopic approaches not only for plant tissues.
Keyphrases
- cell wall
- high resolution
- label free
- raman spectroscopy
- single molecule
- gene expression
- deep learning
- optical coherence tomography
- electronic health record
- big data
- high throughput
- high speed
- data analysis
- ionic liquid
- density functional theory
- convolutional neural network
- machine learning
- photodynamic therapy
- health information
- liquid chromatography