Brush swab as a noninvasive surrogate for tissue biopsies in epigenomic profiling of oral cancer.
Chi T VietXinyu ZhangKe XuGary YuKesava AsamCarissa M ThomasNicholas F CallahanColeen DoanPaul C WalkerKhanh NguyenStephanie C KiddSteve C LeeAnupama GrandhiClint T AllenSimon YoungJames C MelvilleJonathan W ShumDan T VietAlan S HerfordDylan F RodenManuel L GonzalezJiang F ZhongBradley E AouizeratPublished in: Biomarker research (2021)
Our results demonstrate that MC-Seq is an efficient platform for epigenome profiling in cancer biomarker studies, with broader methylome coverage than array-based platforms. Brush swab biopsy provides adequate DNA yield for MC-Seq, and taken together, our findings set the stage for development of a non-invasive methylome quantification technique for oral cancer with high translational potential.