Determination of ochratoxin A in tissues of wild boar (Sus scrofa L.) by enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD).
Giacomo LuciLuigi IntorreGuido FerruzziDanilo ManiLorella GiuliottiCarlo PrettiRosalba TognettiSimone BertiniValentina MeucciPublished in: Mycotoxin research (2017)
Ochratoxin A (OTA) is a secondary toxic metabolite synthesized by Aspergillus or Penicillium species, which can contaminate various crops. The International Agency for Research on Cancer (IARC) classified OTA as a group 2B possible human carcinogen. The aim of the present study was to assess OTA concentrations in tissues of wild boar (Sus scrofa L.) from Tuscany (Italy). Over a period of 2 years, samples of muscle, liver, and kidney from 48 wild boars were collected and concentrations of OTA were determined by enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The highest concentrations of OTA were found in the kidneys of the 48 wild boars analyzed. No difference in concentrations was found based on years of collection and sex while a significantly higher OTA concentration was found in the kidney of the young wild boars with respect to the adult one. Monitoring the quality of meat destined for transformation is a priority in order to decrease the possibility of toxin carry-over to humans. The present study showed that contamination of wild boar meat products by OTA represents a potential emerging source of OTA.
Keyphrases
- high performance liquid chromatography
- solid phase extraction
- simultaneous determination
- tandem mass spectrometry
- mass spectrometry
- genetic diversity
- ms ms
- emergency department
- molecularly imprinted
- escherichia coli
- endothelial cells
- computed tomography
- single molecule
- high resolution
- hydrogen peroxide
- magnetic resonance
- magnetic resonance imaging
- nitric oxide
- squamous cell
- induced pluripotent stem cells
- drinking water