A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide in vitro and in diabetic mice.
Yi-Ru WangFu-Qiang QiaoYu-Wei TanJia-Ling HuAi-Hong ZhangTing LiangXu-Ying LiuHong-Ru SongYan-Fei KangPublished in: Analytical methods : advancing methods and applications (2024)
We designed and prepared probe W-1 for the detection of H 2 O 2 . W-1 showed excellent selectivity for H 2 O 2 and was accompanied by colorimetric signal changes. The excellent linear relationship between fluorescence intensity and H 2 O 2 concentration (0-100 μM) provided favorable conditions for its quantitative detection. In addition, the combination of portable test strips with a smartphone platform provided great convenience for on-site visual detection of H 2 O 2 . Moreover, W-1 possessed targeting mitochondria property and could be applied to image the exogenous and endogenous H 2 O 2 in cells to distinguish normal cells and cancer cells. Lastly, W-1 was used for monitoring the H 2 O 2 fluctuation of the diabetic process in mice, and the results showed an increase in H 2 O 2 levels in diabetes. Therefore, the probe provided a tool for understanding the pathological and physiological mechanisms of diabetes by imaging H 2 O 2 .
Keyphrases
- hydrogen peroxide
- induced apoptosis
- type diabetes
- living cells
- loop mediated isothermal amplification
- cell cycle arrest
- cardiovascular disease
- label free
- cell death
- quantum dots
- high resolution
- single molecule
- nitric oxide
- real time pcr
- cancer therapy
- glycemic control
- gold nanoparticles
- endoplasmic reticulum stress
- deep learning
- fluorescent probe
- reactive oxygen species
- sensitive detection
- signaling pathway
- oxidative stress
- endoplasmic reticulum
- drug delivery
- skeletal muscle
- cell proliferation
- mass spectrometry
- high fat diet induced
- metabolic syndrome
- insulin resistance