Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer.
Xin YuanZhenxiao NiuLang LiuYoujun ZengLin MaZhaogang NieZhen TianDongyun KaiFangteng ZhangGuanyu LiuSiwei LiZhengqiang YuanPublished in: Biosensors (2023)
Intensity interrogation-based surface plasmon resonance imaging (ISPRi) sensing has a simple schematic design and is the most widely used surface plasmon resonance technology at present. In this study, we report the successful development of a novel high-sensitivity ISPRi biosensor and its application for apoptosis detection in cancer cells. By optimizing the excitation wavelength and excitation angle, we achieved a refractive index resolution (RIR) of 5.20 × 10 -6 RIU. Importantly, the biosensor has been tested and validated for high-throughput and label-free detection of activated caspase-3 with its specific inhibitor Z-DEVD-FMK in apoptotic cells. Therefore, this study describes a novel molecular imaging system to monitor apoptosis in cancers for disease diagnosis and/or evaluation of therapeutic efficacy of anti-cancer drugs.
Keyphrases
- label free
- cell cycle arrest
- cell death
- endoplasmic reticulum stress
- induced apoptosis
- oxidative stress
- high resolution
- high throughput
- pi k akt
- loop mediated isothermal amplification
- high intensity
- sensitive detection
- squamous cell carcinoma
- quantum dots
- young adults
- photodynamic therapy
- real time pcr
- mass spectrometry
- lymph node metastasis
- fluorescence imaging