Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.
Wouter EngelenKayleigh M van de WielLenny H H MeijerBedabrata SahaMaarten MerkxPublished in: Chemical communications (Cambridge, England) (2018)
Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.