Analysis of His-Tagged Recombinant Proteins from Ni-NTA and Cu-NTA Coated Surfaces by Direct ESI-MS and DESI-MS.
Roshan JavanshadChristopher James TaylorNiusha DelavariTodd J BarkmanFrederick StullAndre R VenterPublished in: Rapid communications in mass spectrometry : RCM (2023)
The immobilization, purification, release and detection of His-tagged recombinant proteins using immobilized metal affinity surfaces for direct infusion ESI-MS or ambient DESI-MS analyses were successfully demonstrated. Recombinant proteins were purified to allow identification directly out of clarified cell lysate. Biological activities of the recombinant proteins were preserved allowing the investigation of enzymatic activity via mass spectrometry.