Physical and Biological Evaluation of Low-Molecular-Weight Hyaluronic Acid/Fe3O4 Nanoparticle for Targeting MCF7 Breast Cancer Cells.
Hsin-Ta WangPo-Chien ChouPing-Han WuChi-Ming LeeKang-Hsin FanWei-Jen ChangSheng-Yang LeeHaw-Ming HuangPublished in: Polymers (2020)
Low-molecular-weight hyaluronic acid (LMWHA) was integrated with superparamagnetic Fe3O4 nanoparticles (Fe3O4 NPs). The size distribution, zeta potential, viscosity, thermogravimetric and paramagnetic properties of the LMWHA-Fe3O4 NPs were systematically examined. For cellular experiments, MCF7 breast cancer cell line was carried out. In addition, the cell targeting ability and characteristics of the LMWHA-Fe3O4 NPs for MCF7 breast cancer cells were analyzed using the thiocyanate method and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The experimental results showed that the LMWHA-Fe3O4 NPs were not only easily injectable due to their low viscosity, but also exhibited a significant superparamagnetic property. Furthermore, the in vitro assay results showed that the NPs had negligible cytotoxicity and exhibited a good cancer cell targeting ability. Overall, the results therefore suggest that the LMWHA-Fe3O4 NPs have considerable potential as an injectable agent for enhanced magnetic resonance imaging (MRI) and/or hyperthermia treatment in breast cancer therapy.
Keyphrases
- hyaluronic acid
- breast cancer cells
- cancer therapy
- mass spectrometry
- magnetic resonance imaging
- oxide nanoparticles
- drug delivery
- iron oxide
- contrast enhanced
- iron oxide nanoparticles
- computed tomography
- physical activity
- ms ms
- mental health
- liquid chromatography
- single cell
- high resolution
- human health
- cell therapy
- bone marrow
- mesenchymal stem cells